A mediated amperometric biosensor for the determination of uric acid in human serum is described. In the optimized studies, the enzyme electrode prepared by using 20 U/mL uricase and 350 U/mL peroxidase showed optimum performance. Amperometric calibrations were linear over the range of 0.01-2.0 mg/dL, with the former representing the limit of detection. A mean recovery of 100.6% was obtained while various concentrations of uric acid were spiked to human serum samples. The functioning of the biosensor is indifferent to a range of potential interferences. In an interlaboratory study, a good correlation with a conventional spectrometric method was observed. The performance of the biosensor is stable for the first 10 days.
