Determination of uric acid in serum by a mediated amperometric biosensor

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Title:	Determination of uric acid in serum by a mediated amperometric biosensor
Authors:	Tsai WC Wen ST
Contributors:	生科所
Keywords:	amperometric biosensor uric acid mediated uricase HRP
Date:	2006
Issue Date:	2009-11-20 14:48:06 (UTC+8)
Abstract:	A mediated amperometric biosensor for the determination of uric acid in human serum is described. In the optimized studies, the enzyme electrode prepared by using 20 U/mL uricase and 350 U/mL peroxidase showed optimum performance. Amperometric calibrations were linear over the range of 0.01-2.0 mg/dL, with the former representing the limit of detection. A mean recovery of 100.6% was obtained while various concentrations of uric acid were spiked to human serum samples. The functioning of the biosensor is indifferent to a range of potential interferences. In an interlaboratory study, a good correlation with a conventional spectrometric method was observed. The performance of the biosensor is stable for the first 10 days.
Relation:	ANALYTICAL LETTERS Volume: 39 Issue: 5 Pages: 891-901
Appears in Collections:	[Graduate Institute of Biotechnology ] journal articles

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