Naturally occurring chlorophyll derivatives inhibit aflatoxin B-1-DNA adduct formation in hepatoma cells

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Title:	Naturally occurring chlorophyll derivatives inhibit aflatoxin B-1-DNA adduct formation in hepatoma cells
Authors:	Hsu, Ching-Yun Chen, Yue-Hwa Chao, Pi-Yu Chen, Chiao-Ming Hsieh, Ling-Ling Hu, Shene-Pin
Contributors:	食品暨保健營養學系
Keywords:	Aflatoxin B-1 Pheophorbide Glutathione S-transferase GST Chlorophyllide Chlorophyll derivatives
Date:	2008
Issue Date:	2009-11-03 13:28:08 (UTC+8)
Abstract:	The inhibitory effects of four chlorophyll derivatives (chlorophyllide [Chlide] a and b and pheophorbide [Pho] a and b) on aflatoxin B, (AFB(1))-DNA adduct formation, and on the modulation of hepatic glutathione S-transferase (GST) were evaluated in murine hepatoma (Hepa-1) cells. Enzyme-linked immunosorbent assay showed that pretreatment with Chlide or Pho significantly reduced the formation of AFB(1)-DNA adducts, and that Pho was the most potent inhibitor. However, wash-out prior to adding AFB(1) totally eliminated inhibition by Childe and partially eliminated inhibition by Pho. indicating that the inhibitory effect of Chlide, and to some extent Pho, was mediated through direct trapping of AFB(1). Furthermore, spectrophotometric analysis showed that Pho treatment could increase GST activity in Hepa-1 cells. These observations indicate that the chlorophyll derivatives studied may attenuate AFB(1)-induced DNA damage in the Hepa-1 cell by direct trapping of AFB(1). Pho provided additional protection not only by direct trapping, but also by increasing GST activity against hepatic AFB(1) metabolites. (c) 2008 Elsevier B.V. All rights reserved.
Relation:	MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS Volume: 657 Issue: 2 Pages: 98-104
Appears in Collections:	[Department of Food and Nutrition ] journal articles

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