In the contemporary competitive business environment, the significance of intangible assets has grown considerably, given the inherent limitations of the value of physical assets. The biotechnology industry has become a priority sector for the government, and the rapid development of new areas such as tracking applications, in addition to the medical field, indicates that it will continue to grow. The biotechnology industry's core competitive advantages of R&D and innovation, patent protection, and highly specialised human resources have a direct impact on market position and long-term value. The objective of this study is to examine the primary drivers of the value of intangible assets in Taiwanese biotechnology companies and their impact on operational performance. Additionally, the study assesses the value of intellectual assets through financial data analysis and the application of various valuation models. Eight key factors of intangible asset value were initially extracted from a vast array of indicators through factor analysis, and subsequently subjected to Lasso regression analysis in order to filter them as input variables. The findings indicate that R&D investment and growth are the primary determinants of intangible asset value creation in the Taiwanese biotechnology industry. R&D investment and R&D results indicate the company's innovation capability, while growth indicates its future development potential. Furthermore, employee productivity and solvency are also selected. Collectively, these factors affect a company's overall competitiveness and market value, suggesting that a company's market value is not solely dependent on financial capital, but is also significantly influenced by intangible assets.