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請使用永久網址來引用或連結此文件:
https://irlib.pccu.edu.tw/handle/987654321/49459
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| 題名: | Identification of Ipomoea batatas anti-cancer peptide (IbACP)-responsive genes in sweet potato leaves |
| 作者: | Lin, HH (Lin, Hsin-Hung)[ 1 ]
Lin, KH (Lin, Kuan-Hung)
Wu, KF (Wu, Kuan-Fu)
Chen, YC (Chen, Yu-Chi) |
| 貢獻者: | 園生系 |
| 關鍵詞: | Antioxidant enzyme
Plant peptide
Semi-quantitative RT-PCR
Suppression subtractive hybridization
Sweet potato |
| 日期: | 2021-04 |
| 上傳時間: | 2021-04-14 13:15:25 (UTC+8) |
| 摘要: | IbACP, Ipomoea batatas anti-cancer peptide, a sixteen-amino-acid peptide isolated from sweet potato leaves, is capable of mediating a rapid alkalinization of growth media in plant suspension cells. However, the biological roles of IbACP as a defense peptide have not been studied. The objective of this study was to investigate the effect of IbACP on the accumulation of reactive oxygen species (ROS) and the expression of the defense-related genes. IbACP treatment of sweet potato leaves resulted in marked accumulation of both superoxide ion (O-center dot(2)-) and hydrogen peroxide (H2O2). The activity of peroxidase (POD) was significantly enhanced by IbACP treatment, suggesting that high levels of POD antioxidant activity may be used to scavenge the excess H2O2 in sweet potato plants. The IbACP-related genes were identified by suppression subtractive hybridization (SSH), and were then classified and assigned to the following categories: defense, development, metabolism, signaling, gene expression, and abiotic stress. H2O2 acts as a second messenger for gene activation in some of the IbACP-triggered gene expressions. These results demonstrated that IbACP is part of an integrated strategy for genetic regulation in sweet potato. Our work highlights the function of IbACP and its potential use for enhancing stress tolerance in sweet potato, in an effort to improve our understanding of defense-response mechanisms. |
| 關聯: | PLANT SCIENCE 卷冊: 305 文獻號碼: 110849 |
| 顯示於類別: | [園藝暨生物技術學系] 期刊論文
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