文化大學機構典藏 CCUR:Item 987654321/48789
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    题名: UVB Irradiation Induced Cell Damage and Early Onset ofJunbbExpression in Zebrafish
    作者: Chen, RY (Chen, Rui-Yi)
    Lin, CJ (Lin, Chun-Ju)
    Liang, ST (Liang, Sung-Tzu)
    Villalobos, O (Villalobos, Omar)
    Villaflores, OB (Villaflores, Oliver B.)
    Lou, B (Lou, Bao)
    Lai, YH (Lai, Yu-Heng)
    Hsiao, CD (Hsiao, Chung-Der)
    贡献者: 化學系
    关键词: UVB
    zebrafish
    skin
    biomarker
    microarray
    日期: 2020-06
    上传时间: 2020-11-05 10:27:44 (UTC+8)
    摘要: Simple Summary Zebrafish is a good in vivo model to study how skin responds to Ultraviolet B (UVB) irradiation at the cellular, molecular, and whole organism levels. Previous studies showed that zebrafish embryo fin undergoes extensive shrinkage after exposure to UVB irradiation, and this phenotypic change can be assessed using antioxidant drugs. To provide more detailed chronological changes for zebrafish embryos after receiving UVB irradiation, sequential alterations of zebrafish embryos at morphological (fin), cellular (cell death, oxidative stress, immune-response, and marker gene expression) and molecular (microarray screen and real-time RT-PCR assay) levels were examined in this study. The results showed thatjunbbgene expression was activated as early as 3 h post-UVB irradiation, followed by significant elevation of apoptosis around 9 h post-UVB irradiation, neutrophil migration to the wound area approximately 14 h post-UVB irradiation, and activation ofmmpgene expression at around 24 h post-UVB irradiation. These chronological cellular and molecular responses after UVB irradiation in zebrafish provide a basic and fundamental foundation for future line construction and UVB-associated gene validation. Ultraviolet B (UVB) radiation has drawn more attention over these past few decades since it causes severe DNA damage and induces inflammatory response. Serial gene profiling and high throughput data in UVB-associated phenomenon in human cultured cells or full rack of human skin have been investigated. However, results using different tissue models lead to ambiguity in UVB-induced pathways. In order to systematically understand the UVB-associated reactions, the zebrafish model was used, and whole organism gene profiling was performed to identify a novel biomarker which can be used to generate a new mechanistic approach for further screening on a UVB-related system biology. In this study, detailed morphological assays were performed to address biological response after receiving UVB irradiation at morphological, cellular, and molecular levels. Microarray screening and whole genome profiling revealed that there is an early onset expression ofjunbbin zebrafish embryos after UVB irradiation. Also, the identified novel biomarkerjunbbis more sensitive to UVB response thanmmpswhich have been used in mouse models. Moreover, cellular and molecular response chronology after UVB irradiation in zebrafish provide a solid and fundamental mechanism for use in a UV radiation-associated study in the future.
    關聯: ANIMALS 卷冊: 10 期: 6 文獻號碼: 1096
    显示于类别:[化學系所] 期刊論文

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