Leaf necrosis induced by fungal pathogens is one of the most devastating diseases of yam. The objectives of this study were to examine the production of defensive phytohormones and perform a comparative transcriptome analysis between two yam cultivars with different resistance levels againstBotrytis cinereainoculation. After inoculation withB. cinerea, the endogenous ethylene level was found to have accumulated to a higher level in the MH1 resistant cultivar. Meanwhile, expression profiles identified differential defense regulation of ethylene pathway in MH1 (versus susceptible cv. MH3) in response toB. cinerea. A number of ethylene-synthesis and -responsive genes were expressed at higher levels in MH1 than in MH3 after inoculation. Furthermore, ethylene supplementation in MH3 plants by ethephon spraying indeed enhanced the resistance againstB. cinereaandColletotrichum alatae, and elevated the expression ofDaEIN2andDaERF96. Our work improves understanding of defense mechanism and highlights the function of ethylene potentially utilized for yam protection against diseases.
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JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY 卷 30, 期 2, 頁 254 - 264 June 2021
