文化大學機構典藏 CCUR:Item 987654321/45471
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    題名: Overexpression of the Arabidopsis and winter squash superoxide dismutase genes enhances chilling tolerance via ABA-sensitive transcriptional regulation in transgenic Arabidopsis
    作者: Lin, KH (Lin, Kuan-Hung)
    Sei, SC (Sei, Sin-Ci)
    Su, YH (Su, Yu-Huei)
    Chiang, CM (Chiang, Chih-Ming)
    貢獻者: 園生系
    關鍵詞: ABA-sensitive signaling pathway
    AtCBF2
    AtRD29A/B
    chilling tolerance
    Cucurbita moschata
    superoxide dismutase
    日期: 2019-12-02
    上傳時間: 2019-12-13 10:07:42 (UTC+8)
    摘要: The winter squash (Cucurbita moschata, Cm) superoxide dismutase (SOD) CmSOD gene and Arabidopsis thaliana (At)SOD gene were transferred under a ubiquitin promoter into Arabidopsis via Agrobacterium tumefaciens. The expression and amount of SOD and the SOD activities in the AtSOD and CmSOD transgenic lines were significantly higher than those of non-transgenic (NT) plants exposed to 23 or 4 degrees C treatment for 6 similar to 192-h periods. Furthermore, expressions of the cold-inducible gene (AtCBF2) and desiccation-responsible transcription factors (AtRD29A/B) were also activated in all transgenic lines compared to NT plants after chilling treatments. Compared to NT plants under chilling stress, superoxide (center dot O2(-)) accumulation was significantly lower, and chlorophyll (Chl) contents were significantly higher in all transgenic lines with higher SOD activity. Moreover, Arabidopsis seedlings overexpressing AtSOD and CmSOD also displayed greater resistance to chilling and less oxidative injury than NT plants under chilled conditions, indicating that the overexpression of AtSOD and CmSOD in Arabidopsis enhanced chilling tolerance by eliminating center dot O2(-). The expression of AtRD29A was strongly up-regulated only in AtSOD transgenic plants treated with abscisic acid (ABA), while it was repressed in other transgenic plants, indicating ABA-sensitive AtCBF2 and AtRD29A/B transcriptional regulation signaling pathways in transgenic Arabidopsis under chilling conditions.
    關聯: PLANT SIGNALING & BEHAVIOR 卷冊: 14 期: 12 文獻號碼: 1685728
    顯示於類別:[園藝暨生物技術學系] 期刊論文

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