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| 題名: | Microenvironment of saphenous vein graft preservation prior to coronary artery bypass grafting |
| 作者: | Chen, SW (Chen, Shao-Wei)
Chu, Y (Chu, Yen)
Wu, VCC (Wu, Victor Chien-Chia)
Tsai, FC (Tsai, Feng-Chun)
Nan, YY (Nan, Yu-Yun)
Lee, HF (Lee, Hsin-Fu)
Chang, CH (Chang, Chih-Hsiang)
Chu, PH (Chu, Pao-Hsien)
Wu, S (Wu, Semon)
Lin, PJ (Lin, Pyng-Jing) |
| 貢獻者: | 生命科學系 |
| 關鍵詞: | PREVENT IV
SURGERY
FAILURE
BLOOD
O-2 |
| 日期: | 2019-01 |
| 上傳時間: | 2019-06-25 15:37:23 (UTC+8) |
| 摘要: | OBJECTIVES The best preservation solution for a free vascular graft prior to coronary artery bypass grafting (CABG) remains controversial. The aim of this investigation was to evaluate the microenvironment of the human saphenous vein graft when preserved in normal saline (NS) solution or autologous heparinized whole blood (AWB).
METHODS Between January 2014 and December 2014, 21 patients who underwent CABG were enrolled and a total of 162 saphenous vein graft rings were collected. NS and AWB were used to investigate the influence of the microenvironment. The hypoxia, oxidative stress and vascular apoptosis were assayed by western blot, and endothelial integrity was assessed by immunohistochemical analysis.
RESULTS The level of PaO2 in AWB was lower than that in NS (median: 100.5mmHg vs 185.8mmHg, P=0.004). This hypoxic condition led to the production of more hypoxia-inducible factor-1 (median: 60.1% vs 15.1%, P=0.008) and endothelial nitric oxide synthase (median: 52.6% vs 25%, P=0.046) within 30min of preservation time. The fact that higher levels of glutathione peroxidase resulted in the preservation of AWB suggests that it is beneficial to boost the vascular antioxidant defense with lower levels of NOX2. AWB led to increased Bcl-2, reduced cytochrome c and cleaved 85kDa poly ADP-ribose polymerase apoptotic fragments.
CONCLUSIONS We concluded that AWB possesses a microenvironment that is superior to that of NS for saphenous vein graft preservation prior to CABG. |
| 關聯: | Interactive CardioVascular and Thoracic Surgery, Volume 28, Issue 1, January 2019, Pages 71–78 |
| 顯示於類別: | [生命科學系] 期刊論文
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