Human Kinase/Phosphatase-Wide RNAi Screening Identified Checkpoint Kinase 2 as a Cellular Factor Facilitating Japanese Encephalitis Virus Infection

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Title:	Human Kinase/Phosphatase-Wide RNAi Screening Identified Checkpoint Kinase 2 as a Cellular Factor Facilitating Japanese Encephalitis Virus Infection
Authors:	Chan, YL (Chan, Yi-Lin) Liao, CL (Liao, Ching-Len) Lin, YL (Lin, Yi-Ling)
Contributors:	生科系
Keywords:	DNA-DAMAGE RESPONSE CYCLE ARREST REPAIR PROTEINS G(0)/G(1) PHASE HOST FACTORS REPLICATION ACTIVATION PATHWAY CELLS ATM
Date:	2018-05-17
Issue Date:	2019-01-17 15:08:24 (UTC+8)
Abstract:	Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, causes acute encephalitis in humans with high mortality. Not much is known about the interactions between viral and cellular factors that regulate JEV infection. By using a kinase/phosphatase-wide RNAi screening approach, we identified a cell cycle-regulating molecule, checkpoint kinase 2 (CHK2), that plays a role in regulating JEV replication. JEV infection induced G1 arrest and activated CHK2. Inactivation of CHK2 and its upstream ataxia-telangiectasia mutated kinase in JEV-infected cells by using inhibitors reduced virus replication. Likewise, JEV replication was significantly decreased by knockdown of CHK2 expression with shRNA-producing lentiviral transduction. We identified CHK2 as a cellular factor participating in JEV replication, for a new strategy in addressing JEV infection.
Relation:	Frontiers in Cellular and Infection Microbiology 開放取用卷 8, 期 MAY 17 May 2018 論文號碼 142
Appears in Collections:	[Department of Biology ] journal articles

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