Zinc Chloride Exposure Inhibits Brain Acetylcholine Levels, Produces Neurotoxic Signatures, and Diminishes Memory and Motor Activities in Adult Zebrafish

文化大學機構典藏 CCUR > 理工學院 > 理學院 > 化學系所 > 期刊論文 > Item 987654321/41830

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/41830

题名:	Zinc Chloride Exposure Inhibits Brain Acetylcholine Levels, Produces Neurotoxic Signatures, and Diminishes Memory and Motor Activities in Adult Zebrafish
作者:	Sarasamma, S (Sarasamma, Sreeja) Audira, G (Audira, Gilbert) Juniardi, S (Juniardi, Stevhen) Sampurna, BP (Sampurna, Bonifasius Putera) Liang, ST (Liang, Sung-Tzu) Hao, E (Hao, Erwei) Lai, YH (Lai, Yu-Heng) Hsiao, CD (Hsiao, Chung-Der)
贡献者:	化學系所
关键词:	OXIDATIVE STRESS HYPOTHESIS ALZHEIMERS-DISEASE ZNO NANOPARTICLES LOCOMOTOR-ACTIVITY BEHAVIOR GENOTOXICITY COPPER ORYZIAS-LATIPES TOXICITY DANIO-RERIO
日期:	2018-10
上传时间:	2019-01-16 14:24:48 (UTC+8)
摘要:	In this study, we evaluated the acute (24, 48, 72, and 96 h) and chronic (21 days) adverse effects induced by low doses (0.1, 0.5, 1, and 1.5 mg/L) of zinc chloride (ZnCl2) exposure in adult zebrafish by using behavioral endpoints like three-dimensional (3D) locomotion, passive avoidance, aggression, circadian rhythm, and predator avoidance tests. Also, brain tissues were dissected and subjected to analysis of multiple parameters related to oxidative stress, antioxidant responses, superoxide dismutase (SOD), neurotoxicity, and neurotransmitters. The results showed that ZnCl2-exposed fishes displayed decreased locomotor behavior and impaired short-term memory, which caused an Alzheimer's Disease (AD)-like syndrome. In addition, low concentrations of ZnCl2 induced amyloid beta (amyloid ) and phosphorylated Tau (p-Tau) protein levels in brains. In addition, significant induction in oxidative stress indices (reactive oxygen species (ROS) and malondialdehyde (MDA)), reduction in antioxidant defense system (glutathione (GSH), GSH peroxidase (GSH-Px) and SOD) and changes in neurotransmitters were observed at low concentrations of ZnCl2. Neurotoxic effects of ZnCl2 were observed with significant inhibition of acetylcholine (ACh) activity when the exposure dose was higher than 1 ppm. Furthermore, we found that zinc, metallothionein (MT), and cortisol levels in brain were elevated compared to the control group. A significantly negative correlation was observed between memory and acetylcholinesterase (AChE) activity. In summary, these findings revealed that exposure to ZnCl2 affected the behavior profile of zebrafish, and induced neurotoxicity which may be associated with damaged brain areas related to memory. Moreover, our ZnCl2-induced zebrafish model may have potential for AD-associated research in the future.
显示于类别:	[化學系所] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	173	检视/开启

检视Licence

在CCUR中所有的数据项都受到原著作权保护.

数据加载中.....