取四倍體及三倍體異葉銀合歡（K156及K409×K156）無菌播種苗的下胚軸，子葉及節作為試驗材料，MS培養基作為基本培養基。由實驗結果顯示，在下胚軸及子葉的培養之中，不論四倍體或三倍體，誘導癒傷組織能力，以2ppm的2,4-D誘導效果最佳。BA的添加，反而會抑制癒傷組織的產生，而在NAA0.1ppm+BA1ppm時，可促進三倍體異葉銀合歡(K409×K156)子葉，不經由癒傷組織而直接形成多芽體。2ppm的BA最利於四倍體異葉鋇合歡(K156)癒傷組織不定芽的誘導；而三倍體異葉銀合歡(K409×K156)，則以BA3ppm最有效。NAA的參與，可促進癒傷組織的生長，但抑制器官發生。節段之培養，在BA單獨處理及NAA之參與，都會有癒傷組織的形成，而以NAA的參與，對不定芽的誘導較佳。最適合之組合，在四倍體及三倍體異葉銀合歡均為NAA0.01ppm+BA0.5～1ppm。
將形成之莖芽，切下移入含0～3ppm的NAA培養基，都可促進培植體發根，但最適合發根的生長濃度，四倍龍及三倍體異葉銀合歡各不相同，NAA濃度分別為2及0.5ppm。
Callus was induced and plantlets were regenerated by culturing hypocotyls and cotyledons of Leucaena diversifalia, both tetraploid (K156) and tripolid (K409×K156), on Murashige and Skoog's (M5) media containing 2,4-dichlorophenoxyacetic acid (2,4-D) or α-naphthaleneacetic acid (NAA) with 6-benzyladenine (BA). The callus formation was obtained in every explant on MS media with 2 ppm 2,4-B, but inhibited when with BA. Adventitous buds from the cotyledons of triploid (K409×K156) were induced on MS media with 0.1ppm NAA+1ppm BA. Organogenesis via calli was also induced with 2ppm BA for tetraploid (K156) and 3ppm BA for triploid (K409×K156). The best combination of BA+NAA concentration for organogenesis from callus of nodes was 0.5-1ppm BA and 0.1ppm NAA for both tetroploid and triploid Lepsaeiza diversifolia.
Media with 0-3ppm NAA were observed to promote rooting of shoot cuttings. Tetraploids (K156) and. triploids (K409×K156) rooted best on the media with 2ppm and 0.5ppm, respectively.
