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| 題名: | Overexpression of the ascorbate peroxidase gene from eggplant and sponge gourd enhances flood tolerance in transgenic Arabidopsis |
| 作者: | Chiang, CM (Chiang, Chih-Ming)
Chen, C (Chen, Chiu-)
Chen, SP (Chen, Shi-Peng)
Lin, KH (Lin, Kuan-Hung)
Chen, LR (Chen, Li-Ru)
Su, YH (Su, Yu-Huei)
Yen, HC (Yen, His-Cheng) |
| 貢獻者: | 園生系 |
| 關鍵詞: | Flood tolerance
Transgenic
Arabidopsis
Ascorbate peroxidase |
| 日期: | 2017-03 |
| 上傳時間: | 2017-04-19 15:50:28 (UTC+8) |
| 摘要: | Previously, we found that the flood resistance of eggplant (Solanum melongena) and sponge gourd (Luffa cylindrica) enhanced ascorbate peroxidase (APX) activity under flooding, and consequently, both the SmAPX and LcAPX genes were cloned. In this study, the SmAPX and LcAPX genes were transferred under a ubiquitin promoter to Arabidopsis (At) via Agrobacterium tumefaciens. The expression and amount of APX and APX activities of the SmAPX and LcAPX transgenic lines were significantly higher than those of non-transgenic (NT) plants under a waterlogged condition. Furthermore, the SmAPX, LcAPX, At-sucrose synthases (SUS)-1, phosphoenolpyruvate carboxylase (PEPC), and lactate dehydrogenase (LDH) genes were overexpressed in all transgenic Arabidopsis lines after flooding treatment. Compared to NT plants, the malondialdehyde (MDA) contents and H2O2 accumulation were significantly lower, but germination rates were significantly higher in all transgenic lines with higher APX activity, indicating that the overexpression of SmAPX and LcAPX in Arabidopsis could enhance flood tolerance by eliminating H2O2. Moreover, Arabidopsis seedlings overexpressing SmAPX and LcAPX also displayed greater resistance to flooding and less oxidative injury than NT plants subjected to flooding condition. |
| 關聯: | JOURNAL OF PLANT RESEARCH 卷: 130 期: 2 頁碼: 373-386 |
| 顯示於類別: | [園藝暨生物技術學系] 期刊論文
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