Anti-inflammatory effects and mechanisms of the ethanol extract of Evodia rutaecarpa and its bioactive components on neutrophils and microglial cells

文化大學機構典藏 CCUR > 體育運動健康學院 > 技擊運動暨國術學系 > 期刊論文 > Item 987654321/3547

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/3547

题名:	Anti-inflammatory effects and mechanisms of the ethanol extract of Evodia rutaecarpa and its bioactive components on neutrophils and microglial cells
作者:	Han-Chieh Koa Yea-Hwey Wangc Kuo-Tong Lioud Chi-Ming Chenb Chih-Hsiang Chenf Wen-Yen Wange Shiou Change Yu-Chang Houe Kuo-Tung Chenb Chieh-Fu Chenc Yuh-Chiang Shen
贡献者:	國術系
关键词:	Dehydroevodiamine Evodia rutaecarpa Evodiamine Inflammation Microglial cell Neutrophil Reactive oxygen species Nitric oxide (NO) Inducible NO synthetase (iNOS)
日期:	2006-10-17
上传时间:	2010-06-17 09:26:26 (UTC+8)
摘要:	Evodia rutaecarpa is commonly used as an anti-inflammatory drug in traditional Chinese medicine. We previously identified four bioactive compounds (dehydroevodiamine (I), evodiamine (II), rutaecarpine (III), and synephrine (IV)) from the ethanol extract of E. rutaecarpa, but their effects and mechanism(s) of action remain unclear. To study the anti-inflammatory potential and the possible underlying mechanism(s), their effects on phorbol-12-myristate-13-acetate (PMA)- and N-formyl-methionyl-leucyl-phenylalanine (fMLP)-induced reactive oxygen species production in neutrophils was studied, as well as lipopolysaccharide (LPS)-induced nitric oxide (NO) production and inducible NO synthetase (iNOS) expression in microglial cells. The ethanol extract of E. rutaecarpa displayed potent antioxidative effects against both PMA- and fMLP-induced reactive oxygen species production in neutrophils (with IC50 values of around 2.7–3.3 μg/ml). Although less potent than the ethanol extract of E. rutaecarpa, compounds I–IV all concentration-dependently inhibited PMA- and fMLP-induced reactive oxygen species production, with compound IV consistently being the most potent agent among these active components. The antioxidative effects of the ethanol extract of E. rutaecarpa and these compounds were partially due to inhibition (10%–33%) of NADPH oxidase activity, a predominant reactive oxygen species-producing enzyme in neutrophils, and to a minor extent to their direct radical-scavenging properties. The ethanol extract of E. rutaecarpa also inhibited LPS-induced NO production (with an IC50 of around 0.8 μg/ml) and iNOS upregulation in microglial cells that was partially mimicked by compounds I, II, and III, but not compound IV. Our results suggest that the ethanol extract of E. rutaecarpa and its four bioactive components all exhibited anti-inflammatory activities which could be partially explained by their different potentials for inhibiting NADPH oxidase-dependent reactive oxygen species and/or iNOS-dependent NO production in activated inflammatory cells.
關聯:	European Journal of Pharmacology Volume 555, Issues 2-3, 26 P.211-217
显示于类别:	[技擊運動暨國術學系] 期刊論文

文件中的档案:

档案	大小	格式	浏览次数
index.html	0Kb	Unknown	1061	检视/开启

在CCUR中所有的数据项都受到原著作权保护.

数据加载中.....