抗生物質殘留分析常見採行瓊膠擴散試驗，並藉由微生物生長抑制進行篩選；本研究報告檢測試驗系統任務涵蓋初步篩選與後續辨別等二部分。初步篩選檢測研究採用菌膠呈色法之市售許可合格商品PremiRTest 檢測套組，適用動物產品檢體之常用抗生物質殘留檢測，合計九類34種抗生物質涵蓋範圍的檢測結果顯示：乙內醯胺、四環素、胺基配醣苷、巨環素和磺胺劑等類的靈敏度檢測效能足以檢出法定最高殘留容許量濃度。後續辨別檢測研究採用四種培養基菌盤測試法之市售許可合格商品，默克公司抗生物質測試瓊膠培養基的編號暨酸鹼度[I] 10663 pH 6.0，[II] 105270 pH 7.2，[III] 10664 pH 8.0，與[IV] 10664 pH8.0等，並於[I,II,III]與[IV]培養基分別接種枯草桿菌Bacillus subtilis ATCC 6633芽孢與微小球菌Sarcina lutea ATCC 9341等敏感菌株；進行各類抗生物質族群類別之篩選和辨別的檢測結果顯示為四種菌盤敏感性高低順序：乙內醯胺類 IV >I>III >II (或 II> III)；四環素類 I> II> III> IV；胺基配醣苷類 III >II >I >IV；與巨環素類 IV> III> II> I。磺胺劑類檢測另加三甲苄氨嘧啶提高敏感度。 綜合本研究報告結果顯示，抗生物質殘留檢測之篩選與辨別的整合型系統建置應可有效達成實際任務目標。初步篩選型菌膠呈色法適合建置食品供應商的自發性預行檢測，後續辨別型四種培養基菌盤測試法適合建置主管機關的整合型篩選辨別檢測系統；檢測效能靈敏度最佳限值符合衛生署公告之動物用藥殘留標準的殘留容許量，有效確保動物產品符合最高殘留容許量法定標準。
The detection of antibiotics residue is generally analyzed by the growth inhibition ring of sensitive microbial strains revealed on agar plate diffusion test. An integrated efficient detection system of initial screening detection and subsequent differential detection is performed in this research report. The initial screening detection adopts microbial gel coloring method (MGCM) with permitted commercial package on antibiotic residue detection over various animal products. The Premi®Test kit by Dutch DSM food company effectively covers 9 antibiotics classes with 34 drugs of routine usage including beta-lactams, tetracyclines, aminoglycosides, and sulfa drugs. Our detection result with Premi®Test kit revealed superior detection sensitivity on antibiotic residue classes as to be more sensitive than allowed maximal residue quantity of government regulation. Moreover, the subsequent differential detection adopts four plate test (FPT) with permitted microbial media and sensitive microbial strains on antibiotic residue detection over various animal products. Merck antibiotic test agars (MATA) are respectively applied on four plates with catalog numbers of [I] 10663 pH 6.0, [II] 105270 pH 7.2, [III] 10664 pH 8.0, and [IV] 10664 pH 8.0 in which plates [I, II, III] are inoculated with Bacillus subtilis ATCC 6633 and plate [IV] with Sarcina lutea ATCC 9341. Screening and differential detection among several routine antibiotics classes can be accomplished by respective sensitivity ranking pattern of antibiotic residue with FPT inhibitory ring sizes including beta-lactams [IV >I >III >II (or II> III)], tetracyclines [I >II >III >IV], aminoglycosides [III >II >I >IV], macrolides [IV >III >II >I]. In conclusion, the integrated detection system of initial screening detection and subsequent differential detection may be evidently efficient in fulfilling practical goal of antibiotic residue detection over various animal products. Initial screening detection of MACM may be established as voluntary pre-detection by food product suppliers. Subsequent differential detection of FPT may be established as surveillance detection as of integrated detection system by regulatory institutions. Methodology performance of best detection limit is effectively within allowed veterinary drug residue limit supervised by the Department of Health which in turn efficiently assures regulatory compliance on legal standards in allowed maximal antibiotics residue quantity.
