A thorough understanding of the characteristics of transmissivity makes groundwater deterministic models more accurate. These transmissivity data characteristics occasionally possess a complicated spatial variation over an investigated site. This study presents both geostatistical estimation and conditional simulation methods to generate spatial transmissivity maps. The measured transmissivity data from the Dulliu area in Yun-Lin county, Taiwan, is used as the case study. The spatial transmissivity maps are simulated by using sequential Gaussian simulation (SGS), and estimated by using natural log ordinary kriging and ordinary kringing. Estimation and simulation results indicate that SGS can reproduce the spatial structure of the investigated data. Furthermore, displaying a low spatial variability does not allow the ordinary kriging and natural log kriging estimates to fit the spatial structure and small-scale variation for the investigated data. The maps of kriging estimates are smoother than those of other simulations. A SGS multiple realizations has significant advantages over ordinary kriging and even natural log kriging techniques at a site with a high variation in investigated data. These results are displayed in geographic information systems (GIS) as basic information for further groundwater study.