| 摘要: | 半胱胺酸是一種有抗老化功能的胺基酸。半胱氨酸有助於減緩老化症狀、防止心臟病和癌症、增強免疫系統、促進脂肪代謝和肌肉組織的生長、幫助術後癒合、有助於頭髮的生長及防止脫髮等。一些研究表明，青春、健康和生活品質的損失原因可能是因為身體缺乏半胱氨酸。本研究目的在探討半胱胺酸對D-半乳糖誘導 (D-gal) 之老化小鼠的抗氧化功能。實驗鼠 (8週齡，C57BL/6J母鼠) 分為6組，分別為1.控制組、2.老化對照組、及老化加3.低(81.25 mg/kg bw/day)、4.中(162.5 mg/kg bw/day)、5.高(812.5 mg/kg bw/day)劑量半胱胺酸補充劑及6.老化加純化半胱胺酸(50 mg/kg bw/day)組。連續8週每日以皮下注射D-gal (100 mg/kg bw/day) 至小鼠以誘發老化，處理組皆以管餵方式連續8週與D-gal注射期間同步，餵以含半胱胺酸之補充品或純化半胱胺酸。試驗結束後，犧牲動物並取各組織分析superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GR) 之活性並分析Reduced glutathione (GSH), malondialdehyde (MDA)及protein carbonyl (PC) 之濃度。部分組織進一步做成切片後觀察其組織型態之改變。結果顯示，老化控制組之肝臟的穀胱甘肽的濃度(9.18 µg/mg protein)有顯著低於控制組(15.41 µg/mg protein)，胱胺酸補充後，提升肝臟的穀胱甘肽的濃度 (16.41; 14.41; 15.99 µg/mg protein)。胱胺酸補充後，SOD, GST 及 GPx之活性也顯著提升。根據protein carbonyl assay之結果發現半胱胺酸可以幫助修復被D-gal誘導之老鼠之組織損傷。總之，半胱氨酸透過提升組織中的抗氧化劑和降低蛋白質的損害，可以用作抗氧化之補充劑。
Cysteine has been well-known as anti-aging amino acid. Cysteine may help to decrease the effects of aging, protect against heart disease and cancer, boost the immune system, promotes metabolism of fats and production of muscle tissue, healing after surgery, promote hair growth, and prevent hair loss. Some studies suggest that loss of youth, health and quality of life may be partly explained by a deficit in cysteine. The objective of this study is to evaluate the efficacy of cysteine, an anti-aging supporting anti-oxidant, on D-galactose induced aging in an experimental mouse model. Mice were divided into 6 groups, one control group and 5 groups of D-galactose induced aging mice (aging control group, low dose (81.25 mg/kg bw/day), regular dose (162.5 mg/kg bw/day, high dose (812.5 mg/kg bw/day) of cysteine, and one group was administrated by L-cysteine (50 mg/kg bw/day). We used subcutaneous injection of D-galactose (100 mg/kg bw/day) to induce aging in mice, cysteine and L-cysteine is administrated by gavage for 8 weeks. After 8 weeks the mice were sacrificed, tissue samples were analyzed for mean activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST), glutathione reductase (GR). The concentration of reduced glutathione (GSH), malondialdehyde (MDA) and protein carbonyl (PC) were also determined. The histophatological study was performed in order to morphological conformation. The mean level of glutathione from liver was significantly lower in D-galactose control group (9.18 μg/mg protein) than in control group (15.41 μg/mg protein), and increasing after the administration of cysteine containing supplement (16.41; 14.41; 15.99 μg/mg protein). The enzymatic antioxidant activities (SOD, GST and GPx) were increased significantly by cysteine treatment. In addition cysteine repairs the damage of protein peroxidation by lowering the level of protein carbonyl in liver tissue. In conclusion, cysteine can be used as an antioxidant supplement by promoting the antioxidant in tissue and lowering the damage of protein. |
