Biological characterization of cetuximab-conjugated gold nanoparticles in a tumor animal model

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題名:	Biological characterization of cetuximab-conjugated gold nanoparticles in a tumor animal model
作者:	Kao, Hao-Wen Lin, Yi-Yu Chen, Chao-Cheng Chi, Kwan-Hwa Tien, Der-Chi Hsia, Chien-Chung Lin, Wuu-Jyh Chen, Fu-Du Lin, Ming-Hsien Wang, Hsin-Ell
貢獻者:	生科所
關鍵詞:	gold nanoparticle epidermal growth factor receptor cetuximab SPECT radiolabeled
日期:	2014-07-25
上傳時間:	2015-01-22 09:55:09 (UTC+8)
摘要:	Gold nanoparticles (AuNPs) are widely applied to the diagnosis and treatment of cancer and can be modified to contain target-specific ligands via gold-thiolate bonding. This study investigated the pharmacokinetics and microdistribution of antibody-mediated active targeting gold nanoparticles in mice with subcutaneous lung carcinoma. We conjugated AuNPs with cetuximab (C225), an antibody-targeting epidermal growth factor receptor ( EGFR), and then labeled with In-111, which created EGFR-targeted AuNPs. In vitro studies showed that after a 2 h incubation, the uptake of C225-conjugated AuNPs in high EGFR-expression A549 cells was 14.9-fold higher than that of PEGylated AuNPs; furthermore, uptake was also higher at 3.8-fold when MCF7 cells with lower EGFR-expression were used. MicroSPECT/CT imaging and a biodistribution study conducted by using a A549 tumor xenograft mouse model provided evidence of elevated uptake of the C225-conjugated AuNPs into the tumor cells as a result of active targeting. Moreover, the microdistribution of PEGylated AuNPs revealed that a large portion of AuNPs remained in the tumor interstitium, whereas the C225-conjugated AuNPs displayed enhanced internalization via antibody-mediated endocytosis. Our findings suggest that the anti-EGFR antibody-conjugated AuNPs are likely to be a plausible nano-sized vehicle for drug delivery to EGFR-expressing tumors.
關聯:	NANOTECHNOLOGY 卷: 25 期: 29
顯示於類別:	[生物科技研究所 ] 期刊論文

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