重組大腸桿菌用T7 promoter啓動豬病疫苗VP2基因製造，T7 promoter有個lac operon所以必須加入inducer IPTG才能啓動VP2基因。但是IPTC價位太高若想量産豬病疫苗不合乎經濟成本，所以本文探討用IPTC analogue lactose 取代IPTC的可行性，適當控制醱酵條件，經過10-12小時醱酵，加入乳糖取代葡萄糖，再經過4小時，菌體度達20OD600, VP2表現度佔全部蛋白質的15%~20%。
Recombinant E coli uses T7 promoter to produce VP2 gene for porcine vaccine. It is necessary to add inducer IPTG (isopropythio galactoside) to produce VP2 gene because there is a lac operon in the T7 promoter. If we want to mass production VP2 gene, we have to spend much money in inducer IPTG. In this research, we want to study the feasibility of replacing high cost of IPTG by low cost of lactose, The result is that it takes 10~12 hours fermentation then to add lactose. The lactose one is as a carbon source the other is as an inducer. The cell density and VP2 gene expression achieve 20 OD600 and 15~20% respectively after adding lactose 3~4 house.
