海芋 (Zantedeschia aethiopica L.)是重要的經濟球根花卉作物，但在其栽培的過程中常因軟腐菌的為害嚴重影響產量，然而進口種球的價格昂貴，農民大多無法負擔老化及罹病種球的更新，因此建立海芋試管內植株再生途徑生產健康種苗是一個很重要的課題。植物生長的營養需求因植物種類而異，因此本研究第一階段先篩選出適合白花海芋生長之 MS 無機鹽類與蔗糖濃度，將頂芽培養在含有3%、6%蔗糖組合 MS 無機鹽類1/2、全量、兩倍的培養基中，結果顯示芽體在全量的 MS 無機鹽類組合3%蔗糖之培養基中生長情形顯著較佳，四週後芽體相對生長速率91%。在第二階段的誘導芽體形成試驗中，以無菌苗為材料，去除主芽之根莖芽基盤培養於含有3%蔗糖之全量 MS 培養基中添加不同濃度之2,4-二氯苯氧乙酸 (2,4-Dichlorophenoxyacetic acid, 2,4-D) (0、1 mg l-1) 組合噻苯隆 (thidiazuron, TDZ) (0、0.5、1 mg l-1) 和6-苄氨基嘌呤 (6-Benzylaminopurine, BAP) (0、0.5、1 mg l-1) 培養三週後，結果顯示芽基盤培養於單獨含有0.5或1 mg l-1 TDZ 的培養基平均芽體數分別有3.17、3個，但無顯著差異，而芽基盤培養於含有0.5 mg l-1 TDZ 組合0.5 mg l-1 BAP 的培養基平均芽體高度為3.37 cm，較其他濃度顯著，接續將芽體分切繼代至 MS 基本培養基中六個月後馴化出瓶，栽培至3: 1: 1的泥碳土:珍珠石:蛭石介質中，存活率為100%。
Zantedeschia aethiopica L. is an important economic flower crop. But soft rot bacteria in cultivation course seriously damages cutflower production. Most farmers can’t renew the aging and diseased rhizome bulbs due to the high cost of imported bulbs. It is necessary to establish an in vitro plant regeneration system of Z. aethiopica L. Nutrient requirement of plant is dependent on plant species. In the first stage of this study, shoot tip were cultured in MS basal medium (1/2, full, double strength of MS salts) combined with sucrose (3%, 6%). The result showed that full MS basal medium combined with 3% sucrose is optimal for shoot growth, after four weeks relative growth rate of shoot attained to 91%. In the second stage, shoot basal discs derived from in vitro plantlets are used to induce mutiple shoot with application of full MS basal medium combined 3% sucrose supplemented with 2,4-D (0、1 mg l-1) combined with TDZ (0、0.5、1 mg l-1) and BAP (0、0.5、1 mg l-1). The result showed that 0.5 or 1 mg l-1 TDZ can induce multiple shoot formation with the average number 3.17 and 3, irrespectively. The composition of 0.5 mg l-1 TDZ and 0.5 mg l-1 BAP can promote shoot growth with the average height 3.37 cm. The shoots were subcultured to MS medium for rooting. Plantlets can successfully acclimated and the survival rate is 100%.
