| 摘要: | 何首烏 ( Polygonum multiflorum Thunb ) 為蓼科多年生蔓性藤本藥用植物，含有大黃酚、大黃素和大黄素甲醚等蒽醌物質及卵磷脂，據現代醫學研究，其具有抗衰老、補血、強身健體等功效。
本試驗目的為建立何首烏葉片癒傷組織誘導系統，將誘導出的何首烏葉片癒傷組織以生物反應器擴大培養，並利用高效率液相層析（HPLC），進行何首烏葉片癒傷組織內有效成分之分析。以何首烏葉片為培植體，培養於MS培養基中，調節 Auxin (例如：NAA、 2,4-D)、 Cytokinin (例如：BA、 TDZ)生長調節劑之組合，期望能誘導出理想中的癒傷組織，未來可使用生物反應器監控生長條件擴大培養；進階研究則是利用高效液相層析 (HPLC)分析癒傷組織內之有效成分及二次代謝物。研究結果顯示在MS培養基中添加2.0 mg/l NAA與2.0 mg/l TDZ 之組合，可以有效誘導胚原性細胞，且經由HPLC之分析後，發現有二次代謝物大黃酚（chysophanol）的存在。
Polygonum multiflorum Thunb , a perennial medical vine plant of Polygonaceae , contains chysophanol, emodin, physcion etc of anthraquinone derivatives, and lecithin. Base on recent medical researches, functions such as anti-aging, improving red-blood cells development, and maintaining a healthier body, are discovered.
The purpose of this experiment is to study the induction system of Polygonum multiflorum Thunb blade callus, using the bioreactor to expand cultivation of inducted callus, and utilizing High-Performance Liquid Chromatography (HPLC) to analyze the component of the callus. Using Polygonum multiflorum Thunb blade as the explant to cultivate within MS medium, then adjust the ratio of auxin (e.g. NAA, 2, 4-D), cytokinin (e.g. BA, TDZ) in growth modifier, in hope to induce the ideal embryogenic callus, which to be cultivated and growth condition are to be monitored within bioreactor in the future. As a further step, utilizing HPLC to analyze the component of the callus and secondary metabolites is desired.The result shows that by adding 2.0mg/l of NAA and 2.0mg/l of TDZ within MS medium, embryogenic callus can be effective induced. Through HPLC analysis, the existence of secondary metabolites, chysophanol, is discovered. |
