| 摘要: | As a step towards developing an efficient genetically modified organism (GMO) detection system, the present investigation proposes microarray as a prototype for high-throughput detection of pure GMO samples. Common T-DNA regions of the expression cassette such as 35S promoter, 35S terminator, nopaline synthase terminator (NOSt), hygromycin, an kanamycin selection marker genes were detected in the transgenic rice, tomato, and potato developed in our laboratory. For proof-of-concept purposes, market-introduced GM potato, GM maize, and cornflakes were screened. The non-GM potato and maize control detected only the presence of endogenous genes while the GM targets detected the presence of transgenic genes such as CaMV 35S promoter, 35S terminator, NOSt and (n) over bar ptII, pat, cp4 epsps, cry1 Ab genes on chips. Moreover, it was observed that the sensitivity of this system for serially diluted GM potato tubers was up to 0.01-0.002% of the mass fraction. Due to its high accuracy and speed, it is believed that the microarray detection system will play an important role in routine, high-throughput detection of pure GMO samples in the future. |
