在近幾年的活體細胞分析中，細胞電阻抗分析已逐漸成為生物測量的有效方法之 一。生物電阻抗特性藉由細胞不同的電性特性的特徵響應，可以提供比傳統生物病理 組織分析更準確，更深入的細胞電性特性的信息。此外，為瞭解複雜的細胞反應，很 多的細胞參數是需要在單一活體細胞才有辦法做量測的。因此在生物學與醫學的研究 中，研究單一活體細胞特性，已經吸引不少研究人員的投入。 對於單一活體細胞電阻抗量測實驗的第一個步驟是細胞捕獲。因此，單一活體細胞 研究的第一個重要關鍵步驟，就是如何有效率以及自動化的將分離過後懸浮在液體中 的單一細胞捕獲到量測電極的位置來進行細胞電性參數的量測，這也是未來生物晶片 發展一個重要的研究方向。 因此，本計畫提出一種新型電場式單一細胞自動捕獲系統並結合細胞電阻抗量測方 式來建立分析完整細胞電阻抗模型來分析細胞生理與病理上的資訊，以應用於癌症分 析。該電場式單一細胞自動捕獲系統，藉由 OpenCV做影像處理判斷單一細胞與量測電 極相對位置後，再藉由 C8051F320微控制器可程式計算與判別四向細胞捕獲電極中， 哪個電極需要輸入訊號，哪個電極需要關掉訊號，接下來再利用微控制器控制一個四 開關電路，完成單一細胞自動捕獲與量測。 In recent years, the cell impedance analysis of living cells has gradually become one of effective methods of biological measurements. According to electric model function of living cells, the bioelectrical impedance analysis can provide more accurate, more in-depth information of living cell than conventional bio-histopathological analysis. In order to understand the complex cellular responses, the single living cell analysis is used. Therefore, a lot of group is already to study cells characteristics on single living cell level. The first step for single living cells electrical impedance measurements is cell capture. Thus, the first key step of the study of single living cells, is how the efficiency and automation of the separation of a single cell suspension in liquid after capture to the measurement position of the electrode to cell electrical parameter measurement, which is also an important research direction of the future development of biochips. For that reason, this project proposed an automatically electric trapping system for single living cells. Additionally, this device also combined single living cell impedance measurements to establish analysis the complete cell electrical impedance model to analyze the information on cell physiology and pathology, to be applied to the analysis of cancer. Single living cells automatically capture system to be done by OpenCV image processing to determine the relative position of the single cell and the measuring electrode, and then captured by the the C8051F320 microcontroller programmable calculated and discriminant four-way cell electrode, which electrode need input signal, which electrodeneed to turn off the signal, followed by re-use microcontroller to control a four-switch circuit, the completion of a single cell to automatically capture and measurement.
