芘丁酸改質單壁奈米碳管用於乳癌感測

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題名:	芘丁酸改質單壁奈米碳管用於乳癌感測 Single-walled Carbon Nanotubes Modified by Pyrene Butyric Acid for Breast Canser Sensors
作者:	陳聿德 CHEN, YU-DE
貢獻者:	化學工程與材料工程學系奈米材料碩士班
關鍵詞:	奈米碳管 Carbon nanotube
日期:	2013-06
上傳時間:	2013-09-30 11:57:01 (UTC+8)
摘要:	本論文提出了以芘丁酸(N-succinimidyl-1-pyrenebutanoate )來修飾具半導體特性的單壁奈米碳管(SWCNT)，並針對單壁奈米碳管的群聚效應，分別採用甲醇、乙醇、異丙醇三種有機溶劑來分散糾結的單壁奈米碳管，之後再利用傅立葉轉換紅外線光譜儀(FTIR)及拉曼光譜儀(RAMAN)進行結構分析，以及利用紫外可見光光譜儀(UV-Vis)進行溶劑分散效果的判斷。此論文提出的改質方法可使單壁奈米碳管達成分散及改質同步進行，分散在有機溶劑內的單壁奈米碳管同時芘丁酸也經由π-π stacking interactions不可逆的吸附在單壁奈米碳管的管外壁上，再利用光譜儀器分析光譜波峰的變化，不但可以縮短實驗流程，也可以達到使碳管分散及改質的目的。以芘丁酸改質的單壁奈米碳管證實不僅能夠使單壁奈米碳管的分散性更佳，也能夠再進一步藉由類胰島素生長因子第一型受體(IGF1-R)等生物蛋白質鍵結在以芘丁酸改質後的單壁奈米碳管上，最後我們將這些成功改質的單壁奈米碳管運用在生物感測器上，使它扮演生物探針的角色來接收腫瘤病變細胞的訊號，並經由儀器電流的改變來檢測血液中的乳癌細胞。因單壁奈米碳管具有化學穩定性、半導體特性、高表面積及可修飾官能基特性等，故未來盼望能運用此方法研究出高靈敏和高穩定性兼具且能夠即時檢測腫瘤病變細胞的無標記蛋白生物感測器。本研究結果顯示出以異丙醇分散後用芘丁酸改質的單壁奈米碳管效果最明顯，並且非常有可能將此改質後的單壁奈米碳管運用在基於共軛抗體的無標記蛋白生物傳感器上。 This paper presents a with semiconductor properties single-walled carbon nanotubes(SWCNT) to modified pyrene butyric acid (N-succinimidyl-1-pyrenebutanoate) and for SWNTs clustering effect, methanol, ethanol, isopropanol three organic solvents were used to distribute the tangle single-walled carbon nanotubes, after use of Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy (Raman) for structural analysis, and the use of UV-Vis spectrometer (UV-Vis) solvent dispersion effect of the judgment. This paper presents modified SWNTs can reach dispersed and modified simultaneously, single-walled carbon nanotubes dispersed in an organic solvent adsorbed on the pyrene butyric acid via π-π stacking interactions irreversible pipe outer wall of single-walled carbon nanotubes, and then use the spectrometer to analyze the changes in the spectral peaks, can not only shorten the experimental process, you can achieve the purpose of the carbon nanotubes dispersed and modified. Pyrene butyric acid modified single-walled carbon nanotubes confirmed not only be able to make better single-walled carbon nanotube dispersion can further by insulin-like growth factor type 1 receptor (IGF1-R) biological protein binding to pyrene butyric acid modified single-walled carbon nanotubes, we will successfully modified single-walled carbon nanotubes used in biosensor, make it play a biological probe role to receiving the signals of the cells of the tumor lesions, and the change in the current through the instrument to detect breast cancer cells in the blood. Chemical stability due to the single-walled carbon nanotubes, semiconductor properties, high surface area and the modified functional Kitt, look forward to the future use of this method to come up with high sensitivity and high stability both real-time detection of tumor diseased cells label-free protein biosensors. The results of this study showed the most obvious effect of pyrene butyric acid modified isopropanol dispersed SWNTs, and very likely this modified single-walled carbon nanotubes used in conjugated antibody-based label-free protein biosensors.
顯示於類別:	[化學工程與材料工程學系暨碩士班] 博碩士論文

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