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| 題名: | Grape-Seed Procyanidins Inhibit the In Vitro Growth and Invasion of Pancreatic Carcinoma Cells |
| 作者: | Chung, YC (Chung, Yuan-Chiang)
Huang, CC (Huang, Chiu-Chen)
Chen, CH (Chen, Chin-Hui)
Chiang, HC (Chiang, Hua-Che)
Chen, KB (Chen, Kuen-Bao)
Chen, YJ (Chen, Yu-Jen)
Liu, CL (Liu, Chia-Ling)
Chuang, LT (Chuang, Lu-Te)
Liu, M (Liu, Ming)
Hsu, CP (Hsu, Chih-Ping) |
| 貢獻者: | Dept Tourism Management |
| 關鍵詞: | grape-seed procyanidins
pancreatic carcinoma
cell cycle
apoptosis
invasion |
| 日期: | 2012-04 |
| 上傳時間: | 2013-02-21 16:08:08 (UTC+8) |
| 摘要: | Objectives: Grape-seed procyanidins (GSPs) can inhibit cell proliferation and invasiveness in various human cancers. However, the effect of GSP on pancreatic carcinoma cells has not been investigated.
Methods: Pancreatic carcinoma cell lines MIA PaCa-2 and BxPC-3 treated with GSP were assessed for viability by trypan blue exclusion, for cell cycle distribution by flow cytometry, for increased apoptosis by annexin V labeling, for their adhesion and invasion potential by evaluating their ability to penetrate through a matrix gel-coated Boyden chamber, and for changes in the levels of proteins involved in cellular events by immunoblotting.
Results: Grape-seed procyanidin inhibited MIA PaCa-2 and BxPC-3 proliferation in a dose-dependent manner and induced G(1)-phase arrest of the cell cycle in BxPC-3 or mitochondria-mediated apoptosis in MIA PaCa-2. Grape-seed procyanidin also inhibited the adhesion and invasion potential of both cell lines in a dose-dependent manner, which are associated with the suppression of metalloproteases matrix metalloproteinase 9 or 2 (MMP-9 or -2) expression.
Conclusions: Grape-seed procyanidin inhibited the proliferation of pancreatic carcinoma cells by cell cycle blockage or apoptotic induction. The invasiveness was also suppressed by GSP through down-regulation of MMP-2 or MMP-9 in pancreatic carcinoma cells. Grape-seed procyanidin is a potential chemotherapeutic or preventive agent for pancreatic carcinoma. |
| 關聯: | PANCREAS 卷: 41 期: 3 頁數: 447-454 |
| 顯示於類別: | [觀光事業學系暨研究所 ] 期刊論文
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