The objective of this study was to understand the effects of day/night temperature and tuber inoculation on height growth and root collar diameter of Cyclobalanopsis glauca (Querst.) seedlings. All seedlings were raised in phytotron of five different day/night temperature combination (35/30℃, 30/25℃, 25/20℃, 20/15℃ and 15/12℃). Inoculation source used Tuber formosanum and T. aestivum. Seedlings without tuber inoculation were used as control. The results showed that height growth and root collar diameter of seedlings with tuber inoculation were better that those without tuber inoculation. Seedlings at 35/30℃ day/night temperature showed better height growth and root collar diameter. Different growth models were found among tuber treatment and day/night temperature.