本計畫將以纖維性農業廢棄物為基質，經前處理後，透過瘤胃微生物之纖維分解系統模式，有效分解植物纖維成分為還原糖，提供基因改造之木質醋酸菌發酵生產奈米性細菌纖維素之碳源利用。原料將選定各季節盛產之農業廢棄物，先以低耗能之鹼處理，提升纖維生質之可分解度，進一步以瘤胃微生物之酵素分解模式，有效率地對各纖維生質進行分解工作，以轉化廢棄資材為還原糖。透過基因工程技術改造Gluconacetobacter xylinum木質醋酸菌，將異源木糖異構酶（xylose isomerase）表現於G. xylinum中，使其具有代謝木糖發酵生產細菌纖維素之能力；將異源的纖維素結合功能域（cellulose binding domain）表現於G. xylinum中，提高細胞合成細菌纖維素之產量。結合使用木糖異構酶與纖維素結合功能域之雙基因改造G. xylinum重組菌，將能有效利用瘤胃纖維分解系統應用於農業廢棄物資材轉化為永續性綠色材料-細菌纖維素。
The object of this plan will exploit the utilization of cellulosic agricultural waste on bacterial cellulose production. The rumen microbial cellulytic system will apply to degrade the agricultural waste with suitable pretreatment. The reducing sugars converted from cellulose substrate will apply as carbon sources on Gluconacetobacter xylinum growth media to produce bacterial cellulose. The abundant agricultural waste materials from different seasons will select to pretreat with low energy-cost alkali. After increase the degradability of these substrates through the pretreatment, the rumen microbial cellulytic model will utilize to offer a high efficiency cellulytic procedure for reducing sugars converted from agricultural waste. Genetic modification of G. xylinum by gene manipulation techniques will apply to improve some properties and abilities of the recombinant strains. Heterogeneous expression of xylose isomerase in G. xylinum will let the recombinant strain capable of utilizing xylose to produce bacterial cellulose. Heterogeneous expression of cellulose binding domain in G. xylinum is in order to enhance the yield of bacterial cellulose production. Combined use of xylose isomerase and cellulose binding domain genetically engineered recombinant strains, the cellulosic agricultural waste after the treatments of rumen microbial cellulytic system will fermentative convert to sustainable green material - bacterial nanocellulose.
