Cloning, expression and physiological analysis of broccoli catalase gene and Chinese cabbage ascorbate peroxidase gene under heat stress

文化大學機構典藏 CCUR > 理工學院 > 理學院 > 生命科學系 > 期刊論文 > Item 987654321/20979

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题名:	Cloning, expression and physiological analysis of broccoli catalase gene and Chinese cabbage ascorbate peroxidase gene under heat stress
作者:	Lin, KH (Lin, Kuan-Hung) Huang, HC (Huang, Ho-Chang) Lin, CY (Lin, Ching-Yun)
贡献者:	生科所
关键词:	MULTIPLE SEQUENCE ALIGNMENT HIGH-TEMPERATURE STRESS ARABIDOPSIS-THALIANA SUPEROXIDE-DISMUTASE SUBCELLULAR COMPARTMENTS CHLOROPHYLL FLUORESCENCE ANTIOXIDANT METABOLISM LIPID-PEROXIDATION HYDROGEN-PEROXIDE MOLECULAR-CLONING
日期:	2010-12
上传时间:	2011-12-12 09:41:16 (UTC+8)
摘要:	The objectives of this work were to clone the catalase (CAT) gene from broccoli (Brassica oleracea) and the ascorbate peroxidase (APX) gene from Chinese cabbage and measure the regulation of CAT and APX gene expressions under heat-stress conditions. Different genotypes responded differently to heat stress according to their various antioxidant enzymes and physiological parameters. CAT and APX gene expression profiles were well matched with the data for CAT and APX enzyme activities in the broccoli and Chinese cabbage plants, respectively. Full-length of the CAT and APX cDNA were 1,768 and 1,070 bp, respectively. A phylogenetic analysis of CAT and APX indicated that plant CATs and APXs diverged into two major clusters.
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