文化大學機構典藏 CCUR:Item 987654321/20940
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    题名: Porcine pulmonary angiotensin I-converting enzyme-Biochemical characterization and spatial arrangement of the N- and C-domains by three-dimensional electron microscopic reconstruction
    作者: Chen, HL (Chen, Hui-Ling)
    Lunsdorf, H (Luensdorf, Heinrich)
    Hecht, HJ (Hecht, Hans-Juergen)
    Tsai, H (Tsai, Hsin)
    贡献者: 生科所
    关键词: Somatic angiotensin I-converting enzyme
    Pig lung
    N-domain
    C-domain
    Electron microscopy
    3D reconstruction
    日期: 2010-08
    上传时间: 2011-12-09 14:08:29 (UTC+8)
    摘要: The somatic angiotensin I-converting enzyme (sACE; peptidyl-dipeptidase A; EC 3.4.15.1) was isolated from pig lung and purified to homogeneity. The purified enzyme has a molecular mass of about 180 kDa. Upon proteolytic cleavage, two approximately 90 kDa fragments were obtained and identified by amino-terminal sequence analysis as the N- and C-domains of sACE. Both purified domains were shown to be catalytically active. A 2.3 nm resolution model of sACE was obtained by three-dimensional electron microscopic reconstruction of negatively stained sACE particles, based on atomic X-ray data fitting. Our model shows for the first time the relative orientation of the sACE catalytically active domains and their spatial distance. (C) 2010 Elsevier Ltd. All rights reserved.
    显示于类别:[生物科技研究所 ] 期刊論文

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