SPR-based immunosensor for determining staphylococcal enterotoxin A

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Title:	SPR-based immunosensor for determining staphylococcal enterotoxin A
Authors:	Tsai, WC (Tsai, Wen-Chi) Li, IC (Li, Ie-Chin)
Contributors:	生科所
Keywords:	SURFACE-PLASMON RESONANCE SELF-ASSEMBLED MONOLAYERS PIEZOELECTRIC CRYSTAL IMMUNOSENSOR LINKED-IMMUNOSORBENT-ASSAY ENHANCED PERFORMANCE GAD ANTIBODY BIOSENSOR MILK FOOD OUTBREAK
Date:	2009-02
Issue Date:	2011-12-09 11:25:56 (UTC+8)
Abstract:	An immunosensor for determining staphylococcal enterotoxin A (SEA) was developed on the basis of surface plasmon resonance (SPR). The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 16-mercapto-1-hexanol (16-MHA) to 6-mercapto-1-hexanol (6-MCH). The surface functionalized with a 1:20 mixed self-assembled monolayer(SAM) exhibited the best result in terms of SEA detection sensitivity. Through optimization of anti-SEA immobilization, the SPR sensor could detect SEA in phosphate-buffered saline buffer in a linear range from 100 to 1000ng/ml. Furthermore, studies of recovery and matrix effects were performed to evaluate the suitability of the developed immunosensor for directly analyzing SEA in milk samples. Results indicated that the mixed SAM-based SPR immunosensor has the potential for the specific detection of SEA. (C) 2008 Elsevier B.V. All rights reserved.
Appears in Collections:	[Graduate Institute of Biotechnology ] journal articles

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