補充紅麴粉末對脂多醣誘導大鼠器官損傷之效應

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Title:	補充紅麴粉末對脂多醣誘導大鼠器官損傷之效應
Authors:	廖柏翔
Contributors:	生物科技研究所
Keywords:	紅麴粉末脂多醣器官損傷
Date:	2010
Issue Date:	2011-10-31 15:12:36 (UTC+8)
Abstract:	敗血症是一種全身性的發炎反應。目前敗血症的病因不是很清楚，但部分原因和位於革蘭氏陰性細菌細胞壁上的脂多醣（lipopolysaccharide, LPS）或稱內毒素有較大的關係。紅麴為中國千年來傳統的食品發酵菌，它的二次代謝產物monacolin K，目前已被證實是降低膽固醇的生理活性物質。還有些文獻也指出，紅麴的代謝產物以及其還具有抗發炎的功效，如獨特的天然紅麴黃色素monascin和ankaflavin。本研究旨在探討補充紅麴粉末對脂多醣誘導的大鼠器官損傷的效應。本實驗用四週齡Sprague - Dawley（SD）大鼠，隨機分為四組：控制組（補充生理鹽水，腹腔注射生理食鹽水），內毒素組（生理鹽水補充，腹腔注射LPS），低劑量紅麴組（餵飼一倍紅麴粉末，腹腔注射LPS），高劑量紅麴組（補充五倍紅麴粉末，腹腔注射LPS）。經過八週的實驗週期，SD大鼠禁食8小時，然後腹腔注射LPS來誘發敗血症動物模型。注射後18小時，密切觀察並記錄其存活率，收集血液樣本進行血清生化和全血球血液分析。結果顯示，8週後低劑量紅麴組體重顯著低於其他組別且在注射LPS後，白血球 (white blood cells, WBC) 計數也維持在一般的數量並沒有顯著的增加。在LPS組中，血清天門冬胺酸轉胺酶 (aspartate transaminase, AST) 、丙胺酸轉胺酶 (alanine transaminase, ALT) 濃度、BUN等血清生化值均高於對照組。這個結果表明，內毒素注射引起器官損傷的作用。我們的研究結果還表明，低劑量紅麴組對LPS所誘導的器官損傷有保護的作用。然而，高劑量紅麴組雖然血清生化值也稍微降低但和LPS組並無顯著差異。這表明了，適當補充紅麴粉末能有效的保護 LPS誘導的器官損傷。 Sepsis is a systemic inflammatory diseases. The etiology of sepsis is not very clear, but part of the reason is the lipopolysaccharide (LPS) or endotoxin which is in the Gram-negative bacteria's cell wall. The Monascus species is a traditional Chinese food fermentation bacteria, its Secondary metabolites of Monascus monacolin K, prove to be a cholesterol-lowering substance. There are several lines of evidence proven that Monascus fermented products also has anti-inflammatory activity, such as Monascus unique natural yellow pigment monascin and ankaflavin. This study aims to investigate the effect of Monascus powder on LPS-induced organ injury. Fourty male Sprague-Dawley (SD) rats were randomly divided into four groups: Saline-Saline (Saline supplement, Saline intrapertoneal injection), Saline-LPS (Saline supplement, LPS intrapertoneal injection), M1-LPS (one-fold Monascus powder supplement, LPS intrapertoneal injection), M5-LPS (five-fold Monascus powder supplement, LPS intrapertoneal injection). After eight weeks supplementation, SD rats fasting 8 hours and then subjected to receive intrapertoneal LPS administration. At 18 hours post-injection, we calculated the survival rate, and collect blood samples for serum biochemistry and hemocytometric parameters analysis. Following 8 weeks one-fold Monascus powder supplement, the weight gain of M1 group was significant lower than other groups. One-fold dosage Monascus powder supplement also maintain normal WBC number following LPS treatment. The levels of GPT, CRE and BUN of LPS-treated group were higher than that of normal control group. This results suggest that LPS injection cause organ injury in rats. Ours results also show that one-fold Monascus powder attrnuate LPS-induced organ injury in rats. However, fivefold Monascus powder supplement with no protective effect on LPS-induced organ injury. In conclusion, appropriate dosage Monascus powder supplement improves LPS-induced organ injury.
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