以三種山花品種(包括D.batatas,D.alata及D.pseudojaponica)中的六種栽培種的塊莖與葉子為材料進行可溶性蛋白質的抽取，利用免疫染色法發現儲藏性蛋白質(dioscorins)祗存在於塊莖中而不存在於葉子中。在SDS-PAGE膠體上，利用活性染色法證實所有六種栽培種的儲藏性蛋白質都具有碳酸酐酶(carbonic anhydrase)及胰蛋白酶抑制因子(trypsin inhibitor)雙重活性。儲藏性蛋白质利用ConA-peroxidase染色法證實為醣蛋白質。利用原子吸光光度法分析發現從D.batatas Decne所純化的儲藏性蛋白質并不含有鋅原子。而儲藏性蛋白質所具有的碳酸酐酶活性不受5mM acetazolamide及2,6-pyridinedicarboxylic acid抑制。
Dioscorins, the storage proteins of yarn tubers, from six cultivars of three Dioscorea species including D. batatas, D. alata, and D. pseudojaponica were detected in crude extracts of yam tubers but not leaves by immuno stainings on PVDF membrane with a polyclonal antibody which was raised against 28 kDa dioscorin from Dioscorea batatas Decne. It was demonstrated that the dioscorins from the above-mentioned yam species exhibited both carbonic anhydrase (CA) and trypsin inhibitor activities by activity stainings on 15% SDS-PAGE gels. Dioscorins were identified as glycoproteins by ConA-peroxidase activity staining. Dioscorins from D. batatas Decne contained no zinc atoms detectable by atomic absorption spectrophotornetry. Neither 5mM acetazolamide nor 2,6-pyridinedicarboxylic acid inhibited the CA activity of dioscorins.
關聯:
Botanical Bulletin of Academia Sinica 41卷3期 P.191-196
