| 摘要: | 此研究的目的在利用乙醇或水的萃取物分析甘藷不同部位組織的抗氧化和抗癌細胞增生的活性。分析的方法有DPPH 染色法、總多酚類和黃酮類成份測定、清除DPPH 自由基能力、還原力、抑制過氧化物形成能力，和抗癌細胞增殖的活性。在DPPH染色法中，當蔓之酒精萃取液稀釋到6.25mg 乾重/毫升時，仍然具有最高的抗氧化的活性。在所有的萃取物中，蔓之酒精萃取液含有最多的多酚類和黃酮類成份。在清除DPPH自由基能方面，葉子的酒精萃取液含有最高的抗氧化的活性，其次是蔓的水抽液。在還原力的分析上，葉子的水抽液含有最高的還原力，其次是蔓的酒精萃取液。在抑制過氧化物之形成能力上，蔓的酒精萃取液具有最高的抗氧化的活性。在甘藷萃取物抑制癌細胞增生方面，使用的細胞為人類白血球NB4癌細胞，具有最高的抑制能力者為蔓的水抽液 (EC50 為449.6 ±27.73 ug/mL)，其次為塊根的水抽液、葉子的水抽液、塊根的酒精萃取液和葉子的酒精萃取液。由以上結果可知，蔓的酒精萃取液雖具有最高的抗氧化的活性，但不具有抑制癌細胞增生的活性。
The aim of this study is to examine possible antioxidant and antiproliferative activities of the different extracts from sweet potato (Ipomoea batatas [L.] Lam ‘Tainong 57’) organs. DPPH staining, total phenolic compounds and flavonoid content, DPPH radical, reducing power method, FTC method, and cell proliferation were all employed. In the DPPH staining, ethanol extract of vein had the highest radical-scavenging activity when it was diluted to 6.25 mg dry matter/mL. Among all the extracts, the highest amount of total phenolic and flavonoid. compounds was found in the ethanol extract of vein. In the DPPH colorimetric method, it was found that ethanol extract of leaf had the highest radical-scavenging activity, followed by water extract of vein. In the reducing power activity assay, it was found that the water extract of leaf had the highest reducing power activity, followed by ethanol extract of vein. Like phenolic compounds, the highest FTC activity was found in the ethanol extract of vein. The antiproliferative activities aof sweet potato were studied in vitro using human lymphoma NB4 cells, and the following results were found: water extract of vein had the highest antiproliferative activity with an EC50 of 449.6±27.73 ug/mL, followed by water extract of storage root, water extract of leaf, ethanol extract of storage root, and ethanol extract of leaf. Although the ethanol extract of vein showed strong antioxidant activity, it had no antiproliferative activity under the experimental conditions tested. |
