本研究的目的在分析空心菜(Ipomoea aquatica Forsk)不同器官之95%乙醇或水的萃取物的抗氧化和抗細胞增生的活性。分析的方法有DPPH染色法、總多酚類和黃酮類成份測定、清除DPPH自由基之能力、還原力、抑制亞麻油酸過氧化物形成之能力，和抗細胞增殖的活性。在DPPH染色法中，當莖之酒精萃取物稀釋到6.25 mg乾重／毫升時，仍然具有抗氧化的活性；而在此濃度其它萃取物均已顯示不出抗氧化的活性。在所有的萃取物中，莖之酒精萃取物含有最多的多酚類，表現最高之還原力及FTC活性；而在葉子的酒精萃取物中則含有最多的黃酮類成份。在清除DPPH自由基之定量時，莖之酒精萃取物表現最佳，其次是葉子的酒精萃取物。在抑制細胞增生方面，使用的細胞為人類白血球NB4癌細胞，具有最高的抑制能力者為空心菜莖的水抽液（EC50為661.40±3.36 μg dry matter/mL），其次為莖之酒精萃取物和葉子的酒精萃取物。葉子的水萃取物不具有抑制細胞增生的活性(EC50＞1000 μg dry matter/mL)。
The aim of this study as to examine possible antioxidant and antiproliferative activities of 95% ethanol or water extract from water spinach (Ipomoea aquatica Forsk) organs. DPPH staining, total phenolic compounds, total flavonoid content, DPPH radical, reducing power method, FTC method, and inhibition of cancer cell proliferation were employed. Ethanol extract of stem demonstrated a positive effect in DPPH staining when it was diluted to 6.25 mg dry matter/ml. while all other fractions showed no effect at the same dilution. This fraction also had the highest content of the total phenolic compounds, as well as the highest reducing power and FTC activity. Ethanol extract of leaf had the highest amount of flavonoids. Using DPPH colorimetric method, it was found that ethanol extract of stem had the highest radical-scavenging activity, followed by ethanol extract of leaf. The antiproliferative activities of water spinach extracts were studied in vitro using human lymphoma NB4 cells, and the following results were found: water extract of stem had the highest antiproliferative activity with an EC50 of 661.40±3.36 μg dry matter ml., followed by ethanol extract of stem and ethanol extract of leaf The water extract of leaf had the lowest antiproliferative activity (EC50>1000 μg dry matter/mL) under the experimental conditions.
關聯:
Botanical Bulletin of Academia Sinica 46卷2期 P.99-106
