與甘藷產量有關的性狀通常被認為是數量遺傳，顯著受環境因子變異之影響。本研究目的為辨識與甘藷地上部鮮重、根鮮重、根數、根形狀、根皮色與肉色相關之簡單序列重複(ISSR)標誌因子的數量性狀基因座。甘藷'Nancy Hall'(NH)與’臺農27號’(TN27)正、反交所產生120株F1的兩個族群，共篩選100個ISSR引子，根據擴大的、可評分的多型性條帶，辨識出其中18個條帶。所有引子皆於3'端錨接七種雙核苷酸，其中90.7%以簡單simplex標識因子分離；使用simplex與double-simplex之ISSR標識因子建立兩個甘藷的部份連鎖圖譜，每一連鎖群的長度是10.7~149.1centiMorgans(cM)，這些圖譜由NH的37個與TN27的47個標識因子組成，圖譜長度分別為479.8與853.5cM。ISSR標識因子符合六倍體親本對偶基因simplex與duplex的3:1分離比。14與9個與專一性ISSR標識因子連鎖之數量性狀基因座，分別檢測NH×TN27與TN27×NH之性狀，其中22個數量性狀基因座圖繪之標誌因子，對於特殊性狀而言具有特殊性；每個連鎖圖譜中，有兩個QTL圖繪之標誌因子與兩個性狀連鎖，這些標誌因子應可應用於作物改良。
Yield-related traits in sweet potato are generally thought to be quantitative inheritance traits significantly affected by variations in environmental factors. The objective of this study was to identify inter simple sequence repeat (ISSR) markers linked to top weight, root weight, root number, root shape, root skin color, and flesh color in the sweet potato (Ipomoea batatas). Two mapping populations of nearly 120 F1 plants were derived from a reciprocal cross between 'Nancy Hall' (NH) and 'Tainung 27' (TN27). In total, 100 ISSR primers were screened, among which 18 were identified with scorable polymorphic bands that were amplified. All of these primers were anchored at the 3'-end with 7 different types of dinucleotide, and 90.7% of them were segregated as simplex markers. Two partial linkage maps in the sweet potato which use simplex and double-simplex ISSR markers were constructed. The length of each linkage group spanned a very wide range of 10.7~149.1 centiMorgans (cM). These maps consist of 37 NH and 47 TN27 markers with map lengths of 479.8 and 853.5 cM, respectively. The ISSR markers were fitted to a 3:1 ratio of simplex and duplex segregation for the parental alleles, as the sweet potato was expected to be autohexaploid. Fourteen and nine quantitative trait loci (QTL) linked to specific ISSR markers were detected for traits in (NH×TN27) and (TN27×NH), respectively. Twenty-two of these QTL-mapped markers were unique to the specific traits, and two were linked to two traits in each linkage map. These markers appear to be applicable to crop improvement.
