pytochemical study on the constituents of the roots of Symphyotrichum subulatum led to the isolation of three new compounds including two diacetylenes, asterynes A (1) and B (2), and (E)-4-(3-acetoxyprop-1-enyl)-2-methoxyphenyl (S)-2-methylbutanoate (3) along with twelve known compounds. Their structures were elucidated with spectroscopic analyses. Compound 3 showed anti-inflammatory activity on LPS-induced NO production with an EC50 value of 15.0 mu M